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SUPEROXIDE DISMUTASE FAMILY GENES IN WATERMELON AND THEIR RESPONSES TO DIFFERENT ABIOTIC STRESSES

《农业科学与工程前沿（英文）》 2021年 第8卷 第4期   页码 645-658 doi: 10.15302/J-FASE -2020350

摘要：

Superoxide dismutase (SOD) is an important enzyme in the antioxidant system of plants and plays a vital role in stress responses by maintaining the dynamic balance of reactive oxygen species (ROS) concentrations. Genome-wide analysis of the SOD gene family in various plant species has been conducted but little is known about this gene family in watermelon (Citrullus lanatus). Here, eight SOD genes were identified in the watermelon genome and are designated ClCSD1-5, ClFSD1-2 and ClMSD according to their metal cofactors. Phylogenetic analysis shows that SOD proteins from various plant species can be classified into five groups and members in the same group possess the same metal cofactor and similar subcellular localizations. Expression analysis of the ClSOD genes indicates that they had tissue-specific expression patterns with high expression in different tissues including the leaves, flowers and fruit. In addition, the expression of ClSOD genes differed appreciably under salinity, drought and abscisic acid (ABA) treatments, indicating that they may be involved in ROS scavenging under different abiotic stresses via an ABA-dependent signaling pathway. These results lay the foundation for elucidating the function of ClSOD genes in stress tolerance and fruit development in watermelon.

 

关键词： abiotic stress / expression analysis / phylogeny / SOD / superoxide dismutase / watermelon    

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SUPEROXIDE DISMUTASE FAMILY GENES IN WATERMELON AND THEIR RESPONSES TO DIFFERENT ABIOTIC STRESSES

Yong ZHOU, Linjuan OUYANG, Dahu ZHOU, Yicong CAI, Haohua HE

《农业科学与工程前沿（英文）》 2021年 第8卷 第4期   页码 645-658 doi: 10.15302/J-FASE-2020350

摘要： Superoxide dismutase (SOD) is an important enzyme in the antioxidant system of plants and plays a vital role in stress responses by maintaining the dynamic balance of reactive oxygen species (ROS) concentrations. Genome-wide analysis of the gene family in various plant species has been conducted but little is known about this gene family in watermelon ( ). Here, eight genes were identified in the watermelon genome and are designated , and according to their metal cofactors. Phylogenetic analysis shows that SOD proteins from various plant species can be classified into five groups and members in the same group possess the same metal cofactor and similar subcellular localizations. Expression analysis of the genes indicates that they had tissue-specific expression patterns with high expression in different tissues including the leaves, flowers and fruits. In addition, the expression of genes differed appreciably under salinity, drought and abscisic acid (ABA) treatments, indicating that they may be involved in ROS scavenging under different abiotic stresses via an ABA-dependent signaling pathway. These results lay the foundation for elucidating the function of genes in stress tolerance and fruit development in watermelon.

关键词： abiotic stress     expression analysis     phylogeny     SOD     superoxide dismutase     watermelon    

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Genome-wide identification and expression analysis of the β-amylase genes strongly associated with fruitdevelopment, ripening, and abiotic stress response in two banana cultivars

Hongxia MIAO,Peiguang SUN,Yulu MIAO,Juhua LIU,Jianbin ZHANG,Caihong JIA,Jingyi WANG,Zhuo WANG,Zhiqiang JIN,Biyu XU

《农业科学与工程前沿（英文）》 2016年 第3卷 第4期   页码 346-356 doi: 10.15302/J-FASE-2016127

摘要： β-amylase (BAM) is an important enzyme involved in conversion of starch to maltose in multiple biological processes in plants. However, there is currently insufficient information on the BAM gene family in the important fruit crop banana. This study identified 16 genes in the banana genome. Phylogenetic analysis showed that MaBAMs were classified into four subfamilies. Most in each subfamily shared similar gene structures. Conserved motif analysis showed that all identified MaBAM proteins had the typical glyco hydro 14 domains. Comprehensive transcriptomic analysis of two banana genotypes revealed the expression patterns of in different tissues, at various stages of fruit development and ripening, and in responses to abiotic stresses. Most s showed strong transcript accumulation changes during fruit development and late-stage ripening. Some showed significant changes under cold, salt, and osmotic stresses. This finding indicated that might be involved in regulating fruit development, ripening, and responses to abiotic stress. Analysis of five hormone-related and seven stress-relevant elements in the promoters of further revealed that BAMs participated in various biological processes. This systemic analysis provides new insights into the transcriptional characteristics of the genes in banana and may serve as a basis for further functional studies of such genes.

关键词： β-amylase     banana     genome-wide identification     fruit development     abiotic stress    

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The critical role of autophagy in plant responses to abiotic stresses

Yu WANG,Jie ZHOU,Jingquan YU

《农业科学与工程前沿（英文）》 2017年 第4卷 第1期   页码 28-36 doi: 10.15302/J-FASE-2017130

摘要： Autophagy is an evolutionary conserved recycling process in eukaryotes whereby intracellular components are engulfed by autophagosomes, which are subsequently transferred to the vacuoles for further degradation and reuse. In organisms like yeast and metazoans, autophagy is actively engaged during environmental perturbation either by degrading denatured proteins and organelles or by interfacing with stress related signaling molecules. Studies over the last decade have also revealed numerous important mechanisms where autophagy is widely involved in plant abiotic stress responses. Autophagy serves as a pivotal route for nutrient remobilization by the degradation of superfluous or damaged cellular cytoplasmic material and organelles. It is also reported to regulate the accumulation of reactive oxygen species, to maintain the cellular redox balance of plants under stressful conditions. Furthermore, autophagy is essential in regulating cellular toxicity by removing aggregated and/or denatured proteins and thereby improving plant stress tolerance. In this review, recent advances in our understanding of autophagy, along with pathways and regulatory networks through which it influences many aspects of plant growth and development in response to nutrient starvation, oxidative stress, osmotic stress and extreme temperatures are discussed.

关键词： abiotic stresses     autophagy     extreme temperature     nutrient starvation     osmotic stress     oxidative stress    

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Alleviation of cell damage in experimental ANP in rats by administration of chondroitin-sulfate reduces

HE Zhongye, GUO Renxuan, LI Yang, XIE Chengyao, LIU Nan, SONG Wen

《医学前沿（英文）》 2007年 第1卷 第1期   页码 36-40 doi: 10.1007/s11684-007-0007-5

摘要： In order to explore the effects of retrograde infusion of chondroitin-sulfate via the pancreatic duct on cytoprotection and attenuation of oxidative damage during acute necrotic pancreatitis (ANP), male Wistar rats were randomly divided into three groups: A, B (experimental groups) and C (sham operation, control group). The rats in group A was subjected to retrograde injection of 5% sodium taurocholate via the pancreatic duct, and those in group B received chondroitin-sulfate therapy after ANP induction. All rats in three groups were killed at 6 h. The levels of malondialdehyde (MAD), total superoxide dismutase (SOD), glutathione (GSH), adenosine triphosphate (ATP) and serum amylase (SAM) were measured. The morphologic changes in pancreatic tissues were observed. It was found that the level of SAM was increased in group A and group B, with corresponding pathological changes of ANP. The levels of ATP, GSH and SOD in group A were decreased markedly and MDN increased significantly as compared with those in group B (P<0.01). In group B, the histopathologic damage was attenuated to a certain extent in comparison to that in group A. It was concluded that endogenous antioxidants were significantly reduced and lipid peroxidation increased during ANP. Retrograde infusion of chondroitin-sulfate via pancreatic duct could alleviate the pancreatic cell damage as a sort of scavengers of oxygen free radicals.

关键词： control     Wistar     taurocholate     superoxide dismutase     chondroitin-sulfate therapy    

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Leonurine protects ischemia-induced brain injury via modulating SOD, MDA and GABA levels

Shilei ZHENG, Jingru ZHU, Jiao LI, Shuang ZHANG, Yunfei MA

《农业科学与工程前沿（英文）》 2019年 第6卷 第2期   页码 197-205 doi: 10.15302/J-FASE-2018245

摘要：

The present study was designed to investigate the protective effects of leonurine, a compound purified from that is active on ischemic rat behavior and cortical neurons, and explore the underlying mechanism. The general rat activity, cortical neuron morphology, superoxide dismutase (SOD), malondialdehyde (MDA), -aminobutyric acid (GABA) and glutamate decarboxylase 67 (GAD67) levels were measured. We found leonurine significantly improve the general activity of rats in an open-field test, which was associated with attenuated neuronal damage induced by ischemia. Moreover, serum SOD activity was significantly greater, MDA level lower in the leonurine group as compared with ischemia group. In addition, GABA content in the cerebral cortex was significantly greater in high-dose leonurine group. Correspondingly, GAD67 protein level coincided with the GABA level. Taken together, our results demonstrated that leonurine attenuated brain injury during ischemia via antioxidative and anti-excitotoxicity effects by targeting GABA and leonurine might become a useful adjuvant neuroprotective agent.

关键词： cerebral ischemia     GABA     neuroprotection     leonurine     SOD    

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Visible light induces bacteria to produce superoxide for manganese oxidation

《环境科学与工程前沿（英文）》 2023年 第17卷 第2期 doi: 10.1007/s11783-023-1619-y

摘要：

● Term of manganese-oxidizing microorganisms should be reconsidered.

关键词： Mn(II) oxidation     Manganese-oxidizing bacteria     Reactive oxygen species     Mn(III/IV) oxides    

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Expression and bioinformatic analysis of lymphoma-associated novel gene KIAA0372

BAI Xiangyang, TANG Duozhuang, ZHU Tao, SUN Lishi, YAN Lingling, LU Yunping, ZHOU Jianfeng, MA Ding

《医学前沿（英文）》 2007年 第1卷 第1期   页码 93-98 doi: 10.1007/s11684-007-0018-2

摘要： The purpose of this study was to explore the differentially expressed genes in lymph-node cells (LNC) of lymphomas and reactive lymph node hyperplasia, and to perform an initial bioinformatic analysis on a novel gene, KIAA0372, which is highly expressed in the LNC of lymphomas. mRNA extracted from LNC of lymphomas and reactive lymph node hyperplasia were respectively marked with biotin and hybridized with Gene Expression Chips, resulting in differentially expressed genes. Initial bioinformatic analysis was then performed on a novel gene named KIAA0372, whose function has not yet been explored. Its structure and genomic location, its product s physical and chemical properties, subcellular localization and functional domains, were also predicted. Further, a systematic evolution analysis was performed on similar proteins from among several species. Using Gene Expression Chips, many differentially expressed genes were uncovered. Efficient bioinformatic analysis has fundamentally determined that KIAA0372 is an extracellular protein which may be involved in TGF-β signaling. Microarray is an efficient and high throughput strategy for detection of differentially expressed genes. And KIAA0372 is thought to be a potential target for tumor research using bioinformatic analysis.

关键词： bioinformatic analysis     functional     KIAA0372     detection     Microarray    

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Parametric equations for notch stress concentration factors of rib–deck welds under bending loading

《结构与土木工程前沿（英文）》 2021年 第15卷 第3期   页码 595-608 doi: 10.1007/s11709-021-0720-1

摘要： The effective notch stress approach for evaluating the fatigue strength of rib–deck welds requires notch stress concentration factors obtained from complex finite element analysis. To improve the efficiency of the approach, the notch stress concentration factors for three typical fatigue-cracking modes (i.e., root–toe, root–deck, and toe–deck cracking modes) were thoroughly investigated in this study. First, we developed a model for investigating the effective notch stress in rib–deck welds. Then, we performed a parametric analysis to investigate the effects of multiple geometric parameters of a rib–deck weld on the notch stress concentration factors. On this basis, the multiple linear stepwise regression analysis was performed to obtain the optimal regression functions for predicting the notch stress concentration factors. Finally, we employed the proposed formulas in a case study. The notch stress concentration factors estimated from the developed formulas show agree well with the finite element analysis results. The results of the case study demonstrate the feasibility and reliability of the proposed formulas. It also shows that the fatigue design curve of FAT225 seems to be conservative for evaluating the fatigue strength of rib–deck welds.

关键词： notch stress concentration factor     rib–deck weld     parametric analysis     regression analysis     parametric equation    

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Stress-strain relationship with soil structural parameters of collapse loess

SHAO Shengjun, LONG Jiyong, YU Qinggao

《结构与土木工程前沿（英文）》 2008年 第2卷 第2期   页码 151-160 doi: 10.1007/s11709-008-0020-z

摘要： Through the tri-axial shearing tests of unsaturated intact loess and based on the concept of comprehensive soil structural potential, this paper reveals the changing laws of soil structural property under the tri-axial stress conditions and establishes a mathematical expression equation of structural parameters, whereby reflecting the effects of unsaturated loess water content, stress and strain states, which is introduced into the shearing stress and shearing strain relation to obtain the structural stress-strain relation. The tests reveal that the loess dilatancy is of shearing contraction and shearing expansion, whereby indicating that there is a good linear relation between the stress ratio and shearing expansion strain ratio. The larger consolidation confining pressure is, the larger the stress of shearing contraction and expansion critical point is; and the larger water content is, the smaller the strain ratio of shearing contraction and expansion critical point is. Finally, the constitutive model is established to reflect the variation in loess structure, stress-strain softening and hardening, and shearing contraction and shearing expansion features. Through the comparative analysis, the stress-strain curves described by the constitutive relationship are found to be in good conformity with test results, whereby testing the rationality of the model in this paper.

关键词： hardening     shearing expansion     unsaturated     structural property     mathematical expression    

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Coupling of the chemical niche and microbiome in the rhizosphere: implications from watermelon grafting

Yang SONG, Chen ZHU, Waseem RAZA, Dongsheng WANG, Qiwei HUANG, Shiwei GUO, Ning LING, Qirong SHEN

《农业科学与工程前沿（英文）》 2019年 第6卷 第2期   页码 206-206 doi: 10.15302/J-FASE-2018234

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Total stress rapid drawdown analysis of the Pilarcitos Dam failure using the finite element method

Daniel R. VANDENBERGE

《结构与土木工程前沿（英文）》 2014年 第8卷 第2期   页码 115-123 doi: 10.1007/s11709-014-0249-7

摘要： Rapid drawdown is a critical design condition for the upstream or riverside slope of earth dams and levees. A new total stress rapid drawdown method based on finite element analysis is used to analyze the rapid drawdown failure that occurred at Pilarcitos Dam in 1969. Effective consolidation stresses in the slope prior to drawdown are determined using linear elastic finite element analysis. Undrained strengths from isotropically consolidated undrained (ICU) triaxial compression tests are related directly to the calculated consolidation stresses and assigned to the elements in the model by interpolation. Two different interpretations of the undrained strength envelope are examined. Strength reduction finite element analyses are used to evaluate stability of the dam. Back analysis suggests that undrained strengths from ICU tests must be reduced by 30% for use with this rapid drawdown method. The failure mechanism predicted for Pilarcitos Dam is sensitive to the relationship between undrained strength and consolidation stress.

关键词： rapid drawdown     finite element     total stress     slope stability    

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Risk-based probabilistic thermal-stress analysis of concrete arch dams

Narjes SOLTANI, Mohammad ALEMBAGHERI, Mohammad Houshmand KHANEGHAHI

《结构与土木工程前沿（英文）》 2019年 第13卷 第5期   页码 1007-1019 doi: 10.1007/s11709-019-0521-y

摘要： The probabilistic risk of arch dam failure under thermal loading is studied. The incorporated uncertainties, which are defined as random variables, are associated with the most affecting structural (material) properties of concrete and thermal loading conditions. Karaj arch dam is selected as case study. The dam is numerically modeled along with its foundation in three-dimensional space; the temperature and thermal stress distribution is investigated during the operating phase. The deterministic thermal finite element analysis of the dam is combined with the structural reliability methods in order to obtain thermal response predictions, and estimate the probability of failure in the risk analysis context. The tensile overstressing failure mode is considered for the reliability analysis. The thermal loading includes ambient air and reservoir temperature variations. The effect of solar radiation is considered by an increase in the ambient temperatures. Three reliability methods are employed: the first-order second-moment method, the first-order reliability method, and the Monte-Carlo simulation with Latin Hypercube sampling. The estimated failure probabilities are discussed and the sensitivity of random variables is investigated. Although most of the studies in this line of research are used only for academic purposes, the results of this investigation can be used for both academic and engineering purposes.

关键词： arch dams     probabilistic analysis     thermal stress     sensitivity     reliability    

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Numerical analysis of vehicle-bridge coupling vibration concerning nonlinear stress-dependent damping

Pengfei LI; Jinquan ZHANG; Shengqi MEI; Zhenhua DONG; Yan MAO

《结构与土木工程前沿（英文）》 2022年 第16卷 第2期   页码 239-249 doi: 10.1007/s11709-021-0804-y

摘要： Damping is known to have a considerable influence on the dynamic behavior of bridges. The fixed damping ratios recommended in design codes do not necessarily represent the complicated damping characteristics of bridge structures. This study investigated the application of stress-dependent damping associated with vehicle-bridge coupling vibration and based on that investigation proposed the stress-dependent damping ratio. The results of the investigation show that the stress-dependent damping ratio is significantly different from the constant damping ratio (5%) defined in the standard specification. When vehicles travel at speeds of 30, 60, and 90, the damping ratios of the bridge model are 3.656%, 3.658%, and 3.671%, respectively. The peak accelerations using the regular damping ratio are 18.9%, 21.3%, and 14.5% of the stress-dependent damping ratio, respectively. When the vehicle load on the bridge is doubled, the peak acceleration of the mid-span node increases by 5.4 times, and the stress-related damping ratio increases by 2.1%. A corrugated steel-web bridge is being used as a case study, and the vibration response of the bridge is compared with the measured results. The acceleration response of the bridge which was calculated using the stress-dependent damping ratio is significantly closer to the measured acceleration response than that using the regular damping ratio.

关键词： vehicle-bridge vibration system     dynamic analysis     stress-dependent damping     energy dissipation    

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Thermal fluid-structure interaction and coupled thermal-stress analysis in a cable stayed bridge exposed

Nazim Abdul NARIMAN

《结构与土木工程前沿（英文）》 2018年 第12卷 第4期   页码 609-628 doi: 10.1007/s11709-018-0452-z

摘要： In this paper, thermal fluid structure-interaction (TFSI) and coupled thermal-stress analysis are utilized to identify the effects of transient and steady-state heat-transfer on the vortex induced vibration and fatigue of a segmental bridge deck due to fire incidents. Numerical simulations of TFSI models of the deck are dedicated to calculate the lift and drag forces in addition to determining the lock-in regions once using fluid-structure interaction (FSI) models and another using TFSI models. Vorticity and thermal convection fields of three fire scenarios are simulated and analyzed. Simiu and Scanlan benchmark is used to validate the TFSI models, where a good agreement was manifested between the two results. Extended finite element method (XFEM) is adopted to create 3D models of the cable stayed bridge to simulate the fatigue of the deck considering three fire scenarios. Choi and Shin benchmark is used to validate the damaged models of the deck in which a good coincide was seen between them. The results revealed that TFSI models and coupled thermal-stress models are significant in detecting earlier vortex induced vibration and lock-in regions in addition to predicting damages and fatigue of the deck due to fire incidents.

关键词： fire scenario     transient heat transfer     TFSI model     coupled thermal-stress     XFEM    

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标题 作者 时间 类型 操作

SUPEROXIDE DISMUTASE FAMILY GENES IN WATERMELON AND THEIR RESPONSES TO DIFFERENT ABIOTIC STRESSES

期刊论文

SUPEROXIDE DISMUTASE FAMILY GENES IN WATERMELON AND THEIR RESPONSES TO DIFFERENT ABIOTIC STRESSES

Yong ZHOU, Linjuan OUYANG, Dahu ZHOU, Yicong CAI, Haohua HE

期刊论文

Genome-wide identification and expression analysis of the β-amylase genes strongly associated with fruitdevelopment, ripening, and abiotic stress response in two banana cultivars

Hongxia MIAO,Peiguang SUN,Yulu MIAO,Juhua LIU,Jianbin ZHANG,Caihong JIA,Jingyi WANG,Zhuo WANG,Zhiqiang JIN,Biyu XU

期刊论文

The critical role of autophagy in plant responses to abiotic stresses

Yu WANG,Jie ZHOU,Jingquan YU

期刊论文

Alleviation of cell damage in experimental ANP in rats by administration of chondroitin-sulfate reduces

HE Zhongye, GUO Renxuan, LI Yang, XIE Chengyao, LIU Nan, SONG Wen

期刊论文

Leonurine protects ischemia-induced brain injury via modulating SOD, MDA and GABA levels

Shilei ZHENG, Jingru ZHU, Jiao LI, Shuang ZHANG, Yunfei MA

期刊论文

Visible light induces bacteria to produce superoxide for manganese oxidation

期刊论文

Expression and bioinformatic analysis of lymphoma-associated novel gene KIAA0372

BAI Xiangyang, TANG Duozhuang, ZHU Tao, SUN Lishi, YAN Lingling, LU Yunping, ZHOU Jianfeng, MA Ding

期刊论文

Parametric equations for notch stress concentration factors of rib–deck welds under bending loading

期刊论文

Stress-strain relationship with soil structural parameters of collapse loess

SHAO Shengjun, LONG Jiyong, YU Qinggao

期刊论文

Coupling of the chemical niche and microbiome in the rhizosphere: implications from watermelon grafting

Yang SONG, Chen ZHU, Waseem RAZA, Dongsheng WANG, Qiwei HUANG, Shiwei GUO, Ning LING, Qirong SHEN

期刊论文

Total stress rapid drawdown analysis of the Pilarcitos Dam failure using the finite element method

Daniel R. VANDENBERGE

期刊论文

Risk-based probabilistic thermal-stress analysis of concrete arch dams

Narjes SOLTANI, Mohammad ALEMBAGHERI, Mohammad Houshmand KHANEGHAHI

期刊论文

Numerical analysis of vehicle-bridge coupling vibration concerning nonlinear stress-dependent damping

Pengfei LI; Jinquan ZHANG; Shengqi MEI; Zhenhua DONG; Yan MAO

期刊论文

Thermal fluid-structure interaction and coupled thermal-stress analysis in a cable stayed bridge exposed

Nazim Abdul NARIMAN

期刊论文